PRODUCT DETAIL
SUMMARY
The Malaria P.f/P.v Ag Test is a chromatographic immuno assay (CIA) for the rapid qualitative determinatin of P.f/P.v Ag in whole blood. The membrane is pre-coated with antigen capture antibody on the test region. During testing, the red blood cells are lysed releasing Pf HRP- II and pLDH which are allowed to react with the colloidal gold particles coated with Mal-antibody to form antibody-antigen complex. The complex then moves laterally on the membrane and binds to the antibody immobi lized on the membrane producing a pink line on the test region, which indicates a positive result. The presence of the control line demonstrates the test has been performed correctly regardless the presence of P.f/P.v Ag.
SPECIMEN COLLECTION AND HANDLING
1. Clean the area to be lanced with alcohol pad.
2. Squeeze the end of the fingertip and pierce with a sterile lancet provided.
3. Wipe away the first drop of blood with sterile gauze or cotton.
4. Take a capillary pipette (5μl) provided, while gently squeezing the tube, immerse the open end in the blood
drop and then gently release the pressure to draw blood into the capillary pipette to mark line.
5. If specimens are not immediately tested they should be refrigerated at 2 ～ 8℃. For storage periods greater than three days, freezing is recommended. They should be brought to room temperature prior to use.
6. Specimens containing precipitate may yield inconsistent test results. Such specimens must be clarified prior to assaying.
ASSAY PROCEDURE
2.Use the 5μl capillary pipette provided, withdraw the specimen to the mark line. Dispense the specimen to
"S" well.
3.Hold the buffer bottle vertically and add 4 drops of buffer to “A" well. Or using pipette packed in the
separate poly bag, draw and transfer 6 drops of buffer to "A" well.
4.Interpret test results within 10 ~ 20 minutes. Do not interpret after 20 minutes.
